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Abstract

Fusarium vascular wilt disease is one of the most harmful disease that affected broad range of plant species including zucchini (Cucurbita pepo). The objective of the research was to investigate the presence of the phytopathogenic gungus Fusarium oxysporum the causal agent of the disease in zucchini fields. Forty five isolates of F. oxysporum were isolated from four locations in province of Dyala during 2014-2015. Isolate Foq9 was most virulent in the pathogenicity test in vitro. Twenty nine isolates of them amplified their DNA positively with the universal primer of F. oxysporum in the polymerase chain reaction technique (PCR). In vitro significant suppression efficiency were observed of the fungicides Topsin M 70 WP (Tm) and Tecto 500 SC (Tc) and two botanical extract solution extracted from Garlic (Gr) and Ginger (Gn) against the pathogen on the potato sucrose agar (PSA). In greenhouse experiments all of the agents decreased the percentage of disease incidence and severity significantly, while the tetra-inoculum (Tm+Tc+Gr+Gn) was superior which exhibited 0% disease incidence and severity compared to the negative control which was 95%, 79% respectively, also the combined application of the botanical extracts (Gr+Gn) reduced the disease incidence and severity significantly. All of the treatment increased plant growth criteria represented by dry weight of the plant compared to the control.

Keywords

Cucurbita pepo Fusarium oxysporum Garlic extract Ginger extract PCR Tecto Topsen M

Article Details

How to Cite
Hussein, S. N. (2019). Integrated management of the Fusarium vascular wilt disease of Cucurbita pepo in Iraq. Journal of Agricultural and Marine Sciences [JAMS], 23, 40–47. https://doi.org/10.24200/jams.vol23iss0pp40-47
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References

  1. Abd-Elsalam K.A., Ibrahim N.A., Mohmed S.K., Mohmed A.A. and Joseph A.V. 2003. PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data. African Journal of Biotechnology 2 (4):82-85.
  2. Agel, D.S. and R.D. Martyn, 2013. Fusarium wilt of watermelon and other cucurbits. The Plant Health Instructor. doi: 10.1094/PHI-I-2007-0122-01.
  3. Al.Mohammady, F.M. 1990. The Covered Agriculture. Higher Education Press. Iraq. Pp. 400.
  4. Al.Samarrai, G., H. Singh and M. Syarhabil. 2012. Evaluating eco-friendly botanicals (natural plant extracts) as alternatives to synthetic fungicides. Annals of Agricultural and Environmental Medicine, 19(4): 673-676.
  5. Al.Rahmah, A.N., A.A. Mostafa, A. Abdel-Megeed, S.M. Yakout and S. A. Hussein. 2013. Fungicidal activities of certain methanolic plant extracts against tomato phytopathogenic fungi. African Journal of Microbiology Research. 7(6): 517-524. doi: 10.5897/AJMR12.1902
  6. Amini, J. and D.F. Sidovich. 2010. The effects of fungicides on Fusarium oxysporum f. sp. lycopersici associated with Fusarium wilt of tomato. Journal of Plant Protection Research, 50(2): 137-178.
  7. Booth, C. 1971. The Genus Fusarium, Commonwealth Mycological Institute, Kew, Surrey, UK, Pp. 237
  8. Choi, I., J.H. Kim, W.H. Lee, J.H. Park and H.D. Shin. 2015. First report on Fusarium wilt of zucchini caused by Fusarium oxysporum, in Korea. Mycobiology, 43(2): 174-178.
  9. CSS. Central Statistic System. 2012. Iraq.
  10. Domsch, K.H., W. Gams and T. Anderson. 2007. Compendium of Soil Fungi. Second edition. IHW-Verlag, Eching. Pp. 672.
  11. El Khoury, W. and K. Makkouk. 2010. Integrated plant disease management in developing countries. Journal of Plant Pathology. 92 (4):S4.35- S4.42.
  12. Elouaer, M.A. and C. Hannachi. 2012. Seed priming to improve germination and seedling growth of safflower (Carthamus tinctorius) under salt stress. EurAsian Journal of BioSciences. 6: 76-84. doi:10.5053/ejobios.2012.6.0.9
  13. Grau, C.R. and V.L. Radke. 1982. Resistance of soybean cultivar to Sclerotinia sclerotiorum. Journal of Plant disease 66(6): 506-508.
  14. Hassan, A. 1991. Production of Vegetable Crops,1st Edition. Arab House for Publishing and Distribution. Egypt. Pp. 710.
  15. Hussein, S. and K. Juber. 2014. First report of identification Fusarium solani f. sp. cucurbitae race 1 and 2 the causal agent of crown and root rot disease of watermelon in Iraq. Int. J. of Agr. Inno. and Res. 3: 2319-1473.
  16. Hussein, S.N. 2016. Molecular identification and integrated management of the Fusarium f. sp. cucumerinum the causal agent of Fusarium wilt disease of Cucumis sativus L. in Iraq. Journal of Experimental biology and Agricultural sconces 4:389-397. doi: http://dx.doi.org/10.18006/2016.4(4).389.397.
  17. Hussein, S. N. 2014. Some Aspects of Integration to Control Root and Crown Rot Disease of Watermelon. M.Sc. Thesis. Coll. Of Agri. Unv. of Baghdad. Iraq.
  18. Irzykowska, L., J. Bocianowski, A. Waśkiewicz, Z. Weber, Z. Karolewski, P. Goliński, M. Kostecki and W. Irzykowski. 2012. Genetic variation of Fusarium oxysporum isolates forming fumonisin B1 and moniliformin. J. Appl Genet. 53(2): 237-247. doi: 10.1007/s13353-012-0087-z
  19. Jamiołkowska, A., A. Wagner and K. Sawicki. 2011. Fungi colonizing roots of zucchini (Cucurbita pepo L. var. giromontina) plants and pathogenicity of Fusarium spp. to zucchini seedlings. acta agrobotanica. Vol. 64 (1): 73-78.
  20. Lee, Y.S. and C.S. Park. 2001. Fusarium Species of Korea. Plant Pathogens of Korea No. 5. Suwon: National Institute of Agricultural Science and Technology. Korea, Pp. 120.
  21. Logrieco, A., Bottalico A., Mulé G., Moretti A. and G. Perrone.2003. Epidemiology of toxigenic fungi and their associated mycotoxins for some Mediterranean crops. Eur J. Plant Pathol. 109:645-667. doi: 10.1023/A:1026033021542.
  22. Masood A., S. Saeed and N. Iqbal. 2010. Methodology for the evaluation of symptoms severity of mango sudden death syndrome in Pakistan. J. Pak. Bot. 42: 1289-1299.
  23. Mohana, D.C. and K. A. Raveesha. 2007. Anti-fungal evaluation of some plant extracts against some plant pathogenic field and storage fungi. Journal of Agricultural Technology 4(1): 119-137.
  24. Nicholson, G.M. 2007. Fighting the global pest problem: Preface to the special Toxicant issue on insecticidal toxins and their potential for insect pest control. Toxicant, 49(4): 413-422.
  25. Rajput, A.Q., M.H. Arain and M.A. Pathan. 2006. Efficacy of different fungicides against Fusarium wilt of cotton caused by fusarium oxysporum f. sp. vasinfectum. Pak. J. Bot., 38(3): 875-880.
  26. Sahar, P., T. Shahbaz, A. Jabbar, A. Rehman, K. Riaz and A. Hannan, 2013. Chemical and biological management of Fusarium oxysporum f.sp Melongenae. Pak. J. Phytopathol. 25 (15). 155-159
  28. Sanjay, G. and A.K. Tiku. 2009. Botanicals in Pest Management Current Status and Future Perspectives. Biomed Life Sci. Pp. 317.
  29. Schneider, R.W. 1984. Effects of nonpathogeneic strains of Fusarium oxysporum on celery root infection by Fusarium oxysporum f. sp. apii and a novel use of the Lineweaver-Burk double reciprocal plot technique. Phytopathology. 74: 646-653.
  30. Stover R.H. 1962. Fusarium Wilt (Panama Disease) of Bananas and other Musa species. Phytopathological,Wallingford, UK: CAB International. p 4.
  31. Tariq, V.N. and A.C. Magee. 1990. Effect of volatiles from garlic bulb extract on Fusarium oxysporum f. sp. lycopersici. Mycological Research, 94(5) 617-620. doi:10.1016/S0953-7562(09)80662-X