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Abstract

Lactate (LDH) dehydrogenases were studied by electrophoresis to investigate the possibility of isozymic differences in different  stages and organs of B. arabicus embryos, tadpoles and adults. The expression pattern of lactate dehydrogenase (LDH) isozymes was analysed using nondenaturing discontinuous polyacrylamide gel electrophoresis (PAGE). Five LDH isozymes were observed in five different adult tissues. The most cathodal isozyme LDH-5 (A4) showed the strongest activity. All embryonic stages tested displayed the anodic isozymes first. Four LDH isozymes were present in the stages 1-16 (fertilized egg-tail bud stage). The first appearance of the fifth isozyme was detected in stage 17 when the muscular response started. This isozyme showed gradual increase in intensity from stage 17 to stage 43.

 

 

Keywords

Toad embryos tadpoles skeletal muscle LDH Bufo arabicus

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References

  1. AMBU-SAIDI, I. 2002. Staging of Bufo arabicus. M.Sc. Thesis. Sultan Qaboos University, Oman.
  2. ALMEIDA-VAL, V.M., PAULA-SILVA, M.N., CARACIOLO, M.C., MESQUITA, L.; FARIAS, I.P. and VAL, A.L. 1992. LDH isozymes in Amazon fish-III. Distribution patterns and functional properties in Serrasalmidae (Teleostei: Ostriophysi). Comp. Biochem. Physiol. 103B: 119-125.
  3. APPELLA, E. and MARKERT, C.L. 1961. Dissociation of lactate dehydrogenase into subunits with guanidine hydrochloride. Biochem. Biophys. Res. Commun. 6: 171-176.
  4. BA-OMAR, TA; AMBU-SAIDI, I.; AL-BAHRI, S. and AL-KHAYAT, A(2004). Embryonic and larval staging of the toad Bufo arabicus. Zoo .Mid. East. 32: 47-55.
  5. BASU, S.K., GANGULY, S., SARKAR, S.K. and BASU, T.K. 1992. Ontogenetic and comparative analysis of LDH isozymes in the three species on Indian major carp. Ind. J. Exper. Biol. 30: 90-93.
  6. BEIS, A., LAZOU, A. and KONTOGIANNI, E. 1992. Changes in plasma membrane Protein composition during early development of the frog Rana ridibunda. Cellular and Molecular Biol. 38: 513-523.
  7. COPPES, Z.L. 1990. Divegence of duplicate genes in three Scianid species (perciformes) from the South coast of Uruguay. Comp. Biochem Physiol. 96B: 33-39.
  8. COPPES, Z.L. 1984. Isozymes of lactate dehydrogenase in fishes of the superorder Acanthopterygii: an update. Comp. Biochem. Physiol. 97B: 1-8.
  9. GILBERT, S.F. 2001. Ecological developmental biology: developmental biology Meets the real world. Develop. Biol. 233:1-12.
  10. GOLDBERG, E., CUERRIER, J.P. and WARD, J.C. 1969. Lactate dehydrogenase ontogeny, paternal gene activation and tetramer assembly in embryos of brook trout, lake trout and their hybrids. Biochemical Genetics. 2: 335-350.
  11. HRANITZ, J.M. and DIEHL,W.J. 2000. Allozyme variation and population genetic structure during the life history of Bufo woodhousii fowleri (Amphibia: Anura). Biochem. Syst. and Ecol. 28: 15-27.
  12. KAPLAN N, O. 1964. Lactate dehydrogenase: structure and function. Brookhaven Symp. Biol. 17: 131-153.
  13. KUNZ, Y.W. 1973. Changes in lactate dehydrogenase isozyme pattern during the development of Xenopus laevis ( Daudin). Rev. Suisse Zoo. 80(2): 431-446.
  14. MARKERT, C.L., AMET, T.M. and GOLDBERG, E. 1998. Human testis-specific lactate dehydrogenase-C promoter drives over expression of mouse lactate dehydrogenase-1 cDNA in testes of transgenic mice. J. Exp. Zoo. 282: 171-178.
  15. MATHEWS, T.C. 1975. Biochemical polymorphism in populations of the Argentine toad Bufo arenarum. Copeia: 454-465.
  16. MOYER, F.H., SPEAKER, C.B. and WRIGHT, D.A. 1968. Characteristics of lactate dehydrogenase isozymes in amphibians. Ann. N. Y. Acad. Sci. 151: 650-669.
  17. PHILIPP, D.P., CHILDERS, W.F. and WHITT, G.S. 1979. Evolution of patterns of differentialgene expression: a comparison of temporal and spatial patterns of isozyme locus expression in two closely related species (northern large mouth bass, Micropterus salmoides salmoides and small mouth bass, M. dolomieui). J. Exp. Zoo. 210: 473- 488.
  18. ROPSON, I.J., BROWN, D.C. and POWERS, D.A. 1990. Biochemical genetics of Fundulus heteroclitus (L). VI. Geographical variation in the gene frequencies of 15 loci. Evolution. 44: 16-26.
  19. SIMOVICH, M.A. and SASSAMAN, C.A. 1986. Four independent elecrophoretic markers in spade foot toads.
  20. J. Heredity. 77: 410-414.
  21. VONWYL, E. 1983. Expression of the lactate dehydrogenase genes of the Xenopus species and interspecies hybrids during early development. Comp. Biochem. Physiol. 76B: 17-21.