Nutrient Content, in Vitro Ruminal Fermentation Characteristics and Antimethanogenic Potential of Three Algerian Asteraceae Species

Serine Amokrane, Rabah Arhab, Serina Calabro, Raffaella Tudisco, Federico Infascelli, Moufida Aggoun

Abstract


The in vitro rumen fermentation parameters and the antimethanogenic potential of three Asteraceae species: Chamaemelum nobile, Centaurea pulata and Chrysanthemum segetum were determined. Serum bottles containing 200 mg of each plant and 30 ml of the culture medium (artificial saliva plus rumen juice) were incubated for 24 h. After incubation, pH, volatile fatty acid (VFA), ammonia (NH3) and methane (CH4) productions were recorded. Methanogens and protozoa were quantified using  a Real Time PCR technique (qPCR). Cumulative gas productions, in vitro organic matter digestibility and VFA were not significantly affected by the added species when compared to the control (P > 0.05). The effects of Chamaemelum nobile and Chrysanthemum segetum on methane production, NH3 and acetate to propionate ratio (C2:C3) were similar. The two species were able to modulate rumen fermentation to produce significantly lower CH4 concentrations (-24.3% and -27.1%, respectively) compared to the control. C.pulata produced the highest cumulative gas and stimulated the microbial metabolism with an increase in C2:C3 ratio, NH3 and methane production (P < 0.05). No significant effect of the three species on methanogenic Archaea and protozoa was registered (P > 0.05). The three species studied herein show a good potential for mitigating ruminal methane production without any undesirable effects on the main fermentation parameters.


Keywords


Asteraceae; Achaea bacteria; Gas production; Methane; Protozoa and Ruminal fermentation.

Full Text:

PDF

References


Tubiello, F.N., Salvatore, M., Condor Golec, R.D., Ferrara, A., Rossi, S. and Biancalani, R. Agriculture, Forestery and Other Land Use: Emissions by Sources and Removals by Sinks-1990-2011. Analysis. Rapport FAO, ESS working paper N°2, 2014.

Kenny, O., Smyth, T.J., Walsh, D., Kelleher, C.T., Hewage, C.M. and Brunton, N.P. Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts. Food Chemistry., 2014, 161, 79-86.

AOAC. Official Methods of Analysis. (15th Edition). Association of Official Analytical Chemists, Arlington, VA, 1990.

Van Soest, P.J., Robertson, J.B. and Lewis, B.A. Methods for Dietary Fiber, Neutral Detergent Fiber, and Non starch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 1991, 74(10), 3583-3597.

Menke, K.H. and Steingass, H . Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Animal Research Development, 1988, 28, 7-55.

Theodorou, M.K., Williams, B.A., Dhanoa, M.S., Mcallan, A.B. and France, J. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology., 1997, 48, 185-197.

Makkar, HPS. Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds. Small Ruminant Research., 2003, 49, 241-256.

Blümmel, M., Makkar, HPS. and Becker, K. In vitro gas production: a technique revisited. Journal of Animal Physiology and Animal Nutrition., 1997, 77, 24-34.

López, S., Makkar, HPS. and Soliva, C.R. Screening plants and plant products for methane inhibitors. In In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies. Vercoe, P.E., Makkar, HPS., Schlink, A.C. and Dordrecht, 2010.

Maeda, H., Fujimoto, C. and Haruki, Y. Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria. FEMS Immunology and Medical Microbiology., 2003, 39, 81-86.

Denman, S.E., Tomkins, N.W. and McSweeney, C.S. Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane. FEMS Microbiology Ecology., 2007, 62, 313-322.

Denman, S.E. and McSweeney, C.S. Quantitative (Real Time) PCR. InMethods in Gut Microbial Ecology for Ruminants, H. Makkar, H. and McSweeney, C.S. and New York, 2005.

Skillman, L.C.I., Toovey, A.F., Williams, A.J. and Wright, A.D. Development and validation of a real-time PCR method to quantify rumen protozoa and examination of variability between Entodinium populations in sheep offered a hay-based diet. Applied and Environmental Microbiology., 2006, 72(1), 200-6.

Hook, S.E., Steele, M.A., Northwood, K.S., Wright, A.D.G. and McBride, B.W. Impact of High-Concentrate Feeding and Low Ruminal pH on Methanogens and Protozoa in the Rumen of Dairy Cows. Microbial Ecology., 2011, 62, 94-105.

Guimaràtes, R., Barros, L. and Dueñas, M. Nutrients, phytochemicals and bioactivity of wild Roman chamomile: A comparison between the herb and its preparations. Food Chemistry., 2013, 136, 718-725.

Chang, S.C., Lee, M.S., Li, C.H. and Chen, M.H. Dietary fibre content and composition of vegetables in Taiwan area. Asia Pacific Journal of Clinical Nutrition, 1995, 4, 204-210.

Kulivand, M. and Kafilzadeh, F. Correlation between chemical composition, kinetics of fermentation and methane production of eight pasture grasses. Acta scientiarum. Animal Sciences., 2015, 37(1), 9-14.

Arhab, R., Macheboeuf, D., Aggoun, M., Bousseboua, H., Viala, D. and Besle, J.M. Effect of polyethylene glycol on in vitro gas production and digestibility of tannin containing feedstuffs from North African arid zone. Tropical and Subtropical Agroecosystems., 2009, 10(3), 475-486.

Garcia-Gonzalez, R., López, S., Fernandez, M., Bodas, R. and Gonzalez, J.S. Screening the activity of plants and spices for decreasing ruminal methane production in vitro. Animal Feed Science and Technology. 2008, 147, 36-52.

Durmic, Z., Hutton, P., Revell., D.K., Emms, J., Hughes, S. and Vercoe, P.E. In vitro fermentative traits of Australian woody perennial plant species that may be considered as potential sources of feed for grazing ruminants. Animal Feed Science and Technology., 2010, 160, 98-109.

Kamra, D.N., Agarwal, N. and Chaudhary, L.C .Inhibition of ruminal methanogenesis by tropical plants containing secondary compounds. International Congress Series. 2006, 1293, 156-163.

Ungerfield, E.M. Shifts in metabolic hydrogen sinks in the methanogenesis- inhibited ruminal fermentation, Frontiers in Microbiology., 2015, 6(37).

Amokrane, S., Arhab, R., Tudisco, R., Rahab, H., Infascelli, F. and Calabro, S .Effect of Chamaemelum nobile and Chrysanthemum segetum extracts on ruminal methanogenesis, in vitro degradability and methane forming population. International Journal of Advanced Research., 4, 141-154

Khammar, A. and Djeddi S. Pharmacological and Biological Properties of some Centaurea Species. European Journal of Scientific Research., 2012, 84(3), 398-416.

Jayanegara, A., Wina, E., Soliva, C.R., Marquardt, S., Kreuzer, M. and Leiber, F. Dependence of forage quality and methanogenic potential of tropical plants on their phenolic fractions as determined by principal component analysis. Animal Feed Science and Technology., 2011, 16, 231-2430.

Chaves, A.V., Thompson, L.C. and Iwaasa, A.D. Effect of pasture type (alfalfa vs. grass) on methane and carbon dioxide production by yearling beef heifers. Canadian Journal of Animal Science., 2006, 86(3), 409-418.

Stewart, C.S. and Bryant, M.P. The rumen bacteria. In The rumen microbial ecosystem. P.N. Hobson and New York, 1988.

Zhou, Z., Meng, O. and Yu, Z. Effects of Methanogenic Inhibitors on Methane Production and Abundances of Methanogens and Cellulolytic Bacteria in In Vitro Ruminal Cultures. Applied and Environmental Microbiology, 2011, 77(8), 2634-2639.




DOI: http://dx.doi.org/10.24200/squjs.vol25iss1pp10-16

Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Serine Amokrane, Rabah Arhab, Serina Calabro, Raffaella Tudisco, Federico Infascelli, Moufida Aggoun

Creative Commons License
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.

SQUJS 2017-CC BY-ND

This journal and its content is licensed under a Attribution-NoDerivatives 4.0 International.

Flag Counter