Article Sidebar

Submitted
Apr 27, 2017
Published
Jun 1, 2014
Download
PDF
Statistic
Read Counter : 338 Download : 203

Main Article Content

Abstract

Stress due to starvation is not an uncommon phenomenon in fresh-and brackish water fish living in arid environments. This study, a part of a larger investigation on the effects of stress due to starvation on the organ systems of Aphanius dispar, a cyprinodont fish in Oman, reports on the histopathological changes in the liver, using light and transmission electron microscopy.   The hepatocytes showed structural and ultrastructural changes in response to 24, 48, 72 and 96 hours of starvation.  The hepatocytes of the starved fish groups were characterized by a reduction in the size of their cells and their nuclei, chromatin condensation, loss of stored glycogen, large lipid droplets, wide intercellular spaces and the variable sizes of their mitochondria. Swirls of smooth endoplasmic reticulum (SER) were seen in some of the starved fish, which may indicate their involvement in lipid metabolism and the breakdown of glycogen. This paper makes a further contribution to the understanding of the physiology of fish in arid zones.

 

 

 

Keywords

Aphanius dispar fish Liver Hepatocytes Ultrastructure Starvation arid zones.

Article Details

References

  1. . Haas, R. Notes on the ecology of Aphanius
  2. dispar in the Sultanate of Oman. Freshwater Biol, 1982, 12: 89-95.
  3. . Lotan, R. Sodium chloride and water balance in the euryhaline teleost Aphanius dispar (Cyprinodontidae). Zeits. Vergleisch. Physiol. 1969, 65: 455-462
  4. . Lotan, R. Osmotic adjustment in the euryhaline teleost Aphanius dispar (Cyprinodontidae). Zeitsr. Vergleisch. Physiol., 1971, 75: 383-387.
  5. . Plaut, I. 2000. Resting metabolic rate, critical swimming speed, and routine activity of the euryhaline cyprinodontid, Aphanius dispar, acclimated to a wide range of salinities. Physiol. Biochem. Zool., 2000, 73(5): 590-596.
  6. . Storch, V. and Juario, J.V. The effect of starvation and subsequent re-feeding on the hepatocytes of Chanos chanos. Fish Biol., 1983, 23: 95-103.
  7. . Hur, J., Jo, J.H and Park, I.S. Effects of long term starvation on hepatocyte ultrastructure of olive flounder Paralichthys olivaceus. Ichthyol Res., 2006, 53: 306-310.
  8. . Cunnane, S.C. Differential utilization of long chain fatty acids during triacylglycerol depletion. II. rat liver after starvation. Lipids,1988, 23(4): 372-374
  9. . Hung, S.O., Liu, W., Li, H. and Storebaken, T. Effect of starvation on some morphological and biochemical parameters in white sturgeon, Acipenser transmontanus. Aquaculture. 1997, 151: 357-363.
  10. . Kristen, L., Wall, K.L. and Crivello, J. Effects of starvation on liver microsomal P450 activity in juvenile Pleuronectes americanus. Comp. Biochem Physiol. Part C: Pharm. Toxicol. Endocr., 1999, 123: 273-277.
  11. .Deng, L., Zhang, M., Lin, R, Christopher, H.K. and Cheng, C.H.K. Effects of food deprivation on expression of growth hormone receptor and proximate composition in liver of black sea bream Acanthopagrus schlegeli. Comp. Biochem. Physiol. Part B: Biochem. Molecul. Biol., 2004, 137:21-432.
  12. . Morales, E.A., Jiménez, A.B., Hidalgo, M.C., Abellán, E.& Cardenete, G. Oxidative stress and antioxidant defenses after prolonged starvation in Dentex dentex liver. Comp. Biochem. Physiol., 2004, 139: 153-161.
  13. . Ba-Omar, T.A. and Victor, R. and Tobias, D. Some aspects of the anatomy of and histology of the digestive tracts in two sympatric species of freshwater fishes. Sci. Technol. SQU. 2003, 8: 97-106.
  14. . Ba-Omar, T.A. and Victor, R.. Ultrastructural changes in the epithelium of the stomach of Aphanius dispar (Cyprinodontidae), due to stress from starvation. Sci. Technol. SQU, 2000, 5: 1-9.
  15. .Segner, H. and Moller, H. Electron microscopical investigations on starvation induced liver pathology in flounders Platichthys flesus. Mar. Ecol. Prog. Ser.,1984, 19: 193-196.
  16. . Moon TW, Johnston JA. Amino acid transport and inter-conversions in tissues of freshly caught and food-deprived plaice, Pleuronectes platessa L. J. Fish Biol., 1981, 19: 653-663.
  17. . Ba-Omar, T. A., R. Victor and D. B Tobias. Histology of the stomach of Aphanius dispar (Ruppell 1828), a cyprinidont fish, with emphasis on change caused by stress from starvation. Trop. Zoo., 1998, 11: 11-17.
  18. .Ba-Omar, TA; S. Al-Jardani and R. Victor. Effects of pesticide Temephos on the gills of Aphanius dispar (Pisces: Cyprinodontidae). Tissue and Cell. 2011, 43: 29-38.
  19. . Al-Ghanbousi, R. Ba-Omar, T and Victor, R. Effect of deltamethrin on the gills of Aphanius dispar: A microscopic study. Tissue and Cell, 2012, 44: 7-14.