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Abstract

Aflatoxin B1 (AFB1) is a mycotoxin which can cause serious toxicity to animals and humans.  The aim of this study was to investigate the effects of AFB1 in Aphanius dispar fish and measure residues in tissues after in vivo exposure. Aphanius dispar were fed diets containing 50, 100, 150 and 200 µg AFB1/kg for 10, 20 and 30 days. At the end of the experiment, the liver and gills were dissected out and processed for light and electron microscopy. During the experiment, no external changes or unusual behavior were observed in the fish. Histopathological and ultrastructural changes in liver appeared under all four treatments: 50, 100, 150 and 200 µg AFB1/kg. Gill tissues were affected at high doses of 100,150 and 200 µg AFB1/kg. Accumulation of AFB1 residues in liver and gill tissues was found to be related to a dose and duration of exposure.

 

 

Keywords

AFB1 Aphanius dispar Liver Gills Fish Ultrastructure.

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References

  1. IARC. Some naturally occurring substances, food items and constituents, heterocyclic aromatic amines, and mycotoxins. IARC Monographs Evaluation of Carcinogenic Risks in Humans. International Agency for Research on Cancer World Health Organization, Lyon, France. 1993, 56, 489-521.
  2. Santacroce, M.P., Conversano, M.C., Casalino, E., Lia, O., Zizzadoro, C. Centoducati, G. and Crescenzo, G. Aflatoxins in aquatic species: metabolism, toxicity and perspectives. Revs. in Fish Biol. and Fisheries, 2007, 18(1), 99-130.
  3. Elshafie, A.E., Al-Lawatia, T., and Al-Bahry, S.N. Fungi associated with black tea and tea quality in the Sultanate of Oman. Mycopathologia, 1999, 145(2), 89-93.
  4. Elshafie, A.E., Al-Rashdi, T.A., Al-Bahry, S.N and Bakheit, C.S. Fungi and aflatoxins associated with spices in the Sulatanate of Oman. Mycopathologia, 2002, 155, 155-160.
  5. Elshafie, A.E, Al Siyabi, F.M., Salih, F.M., Ba Omar, T., Al Bahry, S.N. and Al Kindi, S. The Mycobiota of herbal drug plants in Oman and possible decontamination by Gamma radiation. Phytopathologia Mediterranea, 2003, 42(2), 149-154.
  6. Williams, D.E., Orner, G., Willard, K.D., Tilton, S., Hendricks., J.D., Pereira, C., Benninghoff, A.D. and Bailey, G.S. Rainbow Trout (Oncorhynchus mykiss) and ultra-low dose cancer studies. Comparative Biochem. Physiol. Part C: Toxicol. & Pharmacol., 2009, 149(2), 175-181.
  7. El-Sayed, Y.S and Khalil, R.H. Toxicity, niochemical effects and residue of Aflatoxin B1 in marine water-reared Sea Bass (Dicentrarchus labrax L.). Food and Chemical Toxicol., 2009, 47(7), 1606-1609.
  8. Hendricks, J.D. Carcinogenicity of aflatoxins in non- mammalian organisms. In: Eaton, D.L., Groopman, J.D. (Eds). The toxicology of aflatoxins: human health, veterinary, and agricultural significance. Academic Press, New York, 1994, pp 103-136.
  9. Anh, T., Grizzle, J., Lovell, R., Manning, B, and Rottinghaus, G. Growth and hepatic lesions of Nile tilapia (Oreochromis niloticus) fed diets containing aflatoxin. Aquaculture, 2002, 212(1), 311-319.
  10. Manning, B.B., Menghe, H.L. and Robinson, E.H. Aflatoxins from moldy corn cause no reductions in channel catfish Ictalurus punctutus performance. J. World Aquaculture Soc., 2005, 36(1), 59-67.
  11. Mishra, H.N., Das, C. A review on biological control and metabolism of aflatoxin. Critical Reviews in Food and Nutrition, 2003, 43(3), 245-264.
  12. Ba-Omar, T., Al-Jardani, S and Victor, R. Effects of Pesticide Temephos on the Gills of Aphanius dispar (Pisces: Cyprinodontidae). Tissue and Cell, 2011, 43, 29-38.
  13. Al-Ghanbousi, R., Ba-Omar, T. and Victor, R. Effect of Deltamethrin on the gills of Aphanius dispar- A microscopic study. Tissue and Cell, 2012, 44(1), 7-14.
  14. Macé, K., Aguilar, F., Wang, J.S., Vautravers, P., Gómez-Lechón, M., Gonzalez, F.J., Groopman, J., Harris, C.C. and Pfeifer, A.M.. Aflatoxin B1-induced DNA adduct formation and p53 mutations in CYP450-expressing human liver cell lines. Carcinogenesis. 1997, 18(7), 1291-1297.
  15. Cagauan, A.G., Tayaban, R.H., Somga, J.R., Bartolome, R.M. Effect of aflatoxin contaminated feeds in Nile tilapia (Oreochromis niloticus L.). In: Remedios, R.B., Mair, G.C., Fitzsimmons, K. Proceedings of the Sixth International Symposium on Tilapia in Aquaculture, 2004, pp. 172-178.
  16. Boonyaratpalin, M., Supamattaya, K., Verakunpiriya, V. and Suprasert, D. Effects of aflatoxin B1 on growth performance, blood components, immune function and histopathological changes in black tiger shrimp (Penaeus monodon Fabricius). Aquaculture Res., 2001, (32), 388-398.
  17. Halver, J.E. Aflatoxicosis and trout hepatoma. In: (L.A. Goldblatt ed.), Aflatoxin: scientific background, control and implications. Academic Press, New York, USA, 1969.
  18. Fatmi, A and Ruby, D. Effect of aflatoxin on total serum protein and liver glycogen of Labeo calbasu, 2011, 6(4), 635-637.
  19. Nunez, J.D. Hendriks and Duimishra, J.R. Ultrastructure of hepato-cellular neoplasm in aflatoxin B1 (AFB1)- initiated rainbow trout (Oncorhynchus mykiss). Toxicological Pathology, 1991, 19(1), 11-21.
  20. Kiessling, K.H. Biochemical mechanisms of action of mycotoxins. Pure Appl. Chem. 1986, 58, 327-338.
  21. Hsieh, D.P.H. Mode of action of mycotoxins. In: Mycotoxins in food, 1987, pp. 149-176. http://thebioscan.in/Journals_PDF/6426-%20AMJAD%20FATMI.pdf
  22. Hussein, H.S. and Brasel, J.M. Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicology, 2001, 167(2), 101-134.
  23. Chou. C.C. and Marth. E.H. Incorporation of [2-14C] acetate into lipids of mink (Mustela risen) liver and intestine during in vitro and in vivo treatment with aflatoxin B1. Appl. and Environmental Microbiology, 1975, 30: 946-950.
  24. Radhika, G., Rajain, P.R., Kizhakkayil, C.G. and Nandiath, K.S. Ultrastructural changes in hepatopancreas of Penaeus mondon Fabricius given aflatoxin B1 diet. Aquaculture Res., 2012, 43, 32-43.
  25. Terao, K. and Ueno, Y. Morphological and functional damage to cells and tissues. In: Toxicology, Biochemistry and Pathology of Mycotoxins, 1978, pp. 189-238.
  26. Sahoo, P.K., Mukherjee, S.C., Jain, A.K., and Mukherjee, A. Histopathological and electron microscopic studies of gills and opisthonephros of Rohu, Labeo rohita to Acute and Subchronic Aflatoxin B1 toxicity. Asian Fisheries Science, 2003, 16(3/4), 257-268.
  27. Roberts, J.R. The pathophysiology and systematic pathology of teleosts. In fish pathology. 1st Ed. 1978, pp. 67-70. Bailliere Tindall, London.
  28. Mallatt, J. Fish gill structural changes induced by toxicants and other irritants; a statistical review. Canadian J. Fisheries and Aquatic Sciences, 1985, 42, 630-648.
  29. Alazemi, B.M., Lewis, J.W. and Andrews, E.B. Gill damage in the freshwater fish, Gnathonemus ptersii (Family: Mormyridae) exposed to selected pollutants: an ultrastructural study. Environmental Technology, 1996, 17(3), 225-238.
  30. Ferguson, H.W. Gills and pseudobranchs. In: Ferguson, H.W., (Ed.), Text book of systemic pathology of fish, 1st ed., Lowa state University press, 1989, pp. 18-20. Amer, Lowa 500/0. Canada.
  31. Hadi, A., Shokr, A. and Alwan, S. Effects of aluminum on the biochemical parameters of fresh waterfish tilapia zillii. J. Appl. Sciences, 2009, 3(1), 33-41.
  32. Figueiredo-Fernandes, A., Ferreira-Cardoso, J.V., Garcia-Santos, S., Monteiro, S.M., Carrola, J., Matos, P., and Fontaínhas-Fernandes, A. Histopathological changes in liver and gill epithelium of Nile tilapia, Oreochromis niloticus exposed to waterborne copper. Brazilian J. Vet. Res., 2007, 27(3), 103-109.
  33. Pawert, M., Mishra, S.K., Chattopadhyay, M., Patro, I.K. Neurotoxic logical effects of deltamethrin on the development of cerebellum of rat. J. Bioscience, 1998, 22(2), 117-130.
  34. Machado, M.R. and Fanta, E. Effect of the organ phosphorous methyl parathion on the branchial epithelium of freshwater fish Metynnis roosevelti. Brazilian Archives of Biology and Technology, 2003, 46(3), 361-372.
  35. Rajeev, R.P., Zhu, X., Lei, W., Han, D., Yang, Y., and Xie, S. Low Levels of Aflatoxin B1 could cause mortalities in juvenile hybrid sturgeon, Acipenser ruthenus male A. Baeri female. Aquaculture Nutrition, 2011, 17(2), 39-47.
  36. Randall, D.J., Connell, D.W., Yang, R. and Wu, S.S. Concentrations of persistent lipophilic compounds in fish are determined by exchange across the gills, not through the food chain. Chemosphere, 1998, 37, 1263-1270.