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Abstract
Improved and simplified HPLC conditions for the determination and quantification of both the steroidal glycoalkaloid (solamargine) and its aglycone (solasodine) are described. The best isocratic conditions were developed using a C18 column and methanol in combination with an ammonium dihydrogen phosphate buffer. The isocratic conditions were shown to be more reproducible, less time consuming and to give sharper peaks (better separation). The effects of adjusting solvent: buffer ratio, buffer pH and buffer molarity were evaluated.
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References
- CARMAN, A.S., KUAN, S.S., WARE, G.M., FRANCIS, O.J. and KIRSCHENHENHEUTER, G.P. 1986. Rapid High-Performance Liquid Chromatographic Determination of the Potato Glycoalkaloids α-Solanine and α-Chaconine. J. Agricultural and Food Chemistry, 34(2): 279-282.
- CHERKAOUI, S., BEKKOUCHE, K., CHRISTEN, P. and VEUTHEY, J.L. 2001. Non-Aqueous Capillary Electrophoresis with Diode Array and Electrospray Mass Spectrometric Detection for the Analysis of Selected Steroidal Alkaloids in Plant Extracts. J. Chromatography A, 922(1-2): 321-328.
- CRABBE, P.G. and FRYER, C. 1980. Rapid Quantitative Analysis of Solasodine, Solasodine Glycosides and Solasodiene by High-Pressure Liquid Chromatography. J. Chromatography A, 187(1): 87-100.
- DINAN, L., HARMATHA, J. and LAFONT, R. 2001. Chromatographic Procedures for the Isolation of Plant Steroids. J. of Chromatography A. 935(1-2): 105-123.
- EANES, R.C., TEK, N., KIRSORY, O., FRARY, A., DOGANLAR, S. and ALMEIDA, A.E. 2008. Development of Practical HPLC Methods for the Separation and Determination of Eggplant Steroidal Glycoalkaloids and their Aglycones. J. Liquid Chromatography & Related Technologies. 31(7): 984-1000.
- ELTAYEB, E.A., AL-ANSARI, A.S. and RODDICK, J.G. 1997. Changes in the Steroidal Alkaloid Solasodine During Development of Solanum Nigrum and Solanum Incanum. Phytochemistry, 46(3): 489-494.
- FRIEDMAN, M. and DAO, L. 1992. Distribution of Glycoalkaloids in Potato Plants and Commercial Potato Products. J. Agricultural and Food Chemistry, 40(3): 419-423.
- JACOB, A. and MALPATHAK, N. 2005. Plantlet Regeneration Enhances Solasodine Productivity in Hairy Root Cultures of <i> Solanum Khasianum </i> Clarke. In Vitro Cellular & Developmental Biology - Plant, 41(3): 291-295.
- KREFT, S., ZEL, J., PUKL, M., UMEK, A. and STRUKELJ, B. 2000. Non-Aqueous Capillary Electrophoresis for the Simultaneous Analysis of Solasodine and Solasonine. Phytochemical Analysis, 11(1): 37-40.
- KURONEN, P., VÄÄNÄNEN, T. and PEHU, E. 1999. Reversed-Phase Liquid Chromatographic Separation and Simultaneous Profiling of Steroidal Glycoalkaloids and their Aglycones. J. of Chromatography A, 863(1): p. 25-35.
- LEE, M.-H., CHENG, J.-J., LIN, C.-Y., CHEN, Y.-J. and LU, M.-K. 2007. Precursor-Feeding Strategy for the Production of Solanine, Solanidine and Solasodine by a Cell Culture of Solanum Lyratum. Process Biochemistry, 42(5): 899-903.
- TRIVEDI, P. and PUNDARIKAKSHUDU, K. 2007. Novel TLC Densitometric Method for Quantification of Solasodine in Various <i> Solanum </i> Species, Market Samples and Formulations. Chromatographia, 65(3): 239-243.
- WEISSENBERG, M. 2001. Isolation of Solasodine and other Steroidal Alkaloids and Sapogenins by Direct Hydrolysis-Extraction of Solanum Plants or Glycosides Therefrom. Phytochemistry, 58(3): 501-508.
References
CARMAN, A.S., KUAN, S.S., WARE, G.M., FRANCIS, O.J. and KIRSCHENHENHEUTER, G.P. 1986. Rapid High-Performance Liquid Chromatographic Determination of the Potato Glycoalkaloids α-Solanine and α-Chaconine. J. Agricultural and Food Chemistry, 34(2): 279-282.
CHERKAOUI, S., BEKKOUCHE, K., CHRISTEN, P. and VEUTHEY, J.L. 2001. Non-Aqueous Capillary Electrophoresis with Diode Array and Electrospray Mass Spectrometric Detection for the Analysis of Selected Steroidal Alkaloids in Plant Extracts. J. Chromatography A, 922(1-2): 321-328.
CRABBE, P.G. and FRYER, C. 1980. Rapid Quantitative Analysis of Solasodine, Solasodine Glycosides and Solasodiene by High-Pressure Liquid Chromatography. J. Chromatography A, 187(1): 87-100.
DINAN, L., HARMATHA, J. and LAFONT, R. 2001. Chromatographic Procedures for the Isolation of Plant Steroids. J. of Chromatography A. 935(1-2): 105-123.
EANES, R.C., TEK, N., KIRSORY, O., FRARY, A., DOGANLAR, S. and ALMEIDA, A.E. 2008. Development of Practical HPLC Methods for the Separation and Determination of Eggplant Steroidal Glycoalkaloids and their Aglycones. J. Liquid Chromatography & Related Technologies. 31(7): 984-1000.
ELTAYEB, E.A., AL-ANSARI, A.S. and RODDICK, J.G. 1997. Changes in the Steroidal Alkaloid Solasodine During Development of Solanum Nigrum and Solanum Incanum. Phytochemistry, 46(3): 489-494.
FRIEDMAN, M. and DAO, L. 1992. Distribution of Glycoalkaloids in Potato Plants and Commercial Potato Products. J. Agricultural and Food Chemistry, 40(3): 419-423.
JACOB, A. and MALPATHAK, N. 2005. Plantlet Regeneration Enhances Solasodine Productivity in Hairy Root Cultures of <i> Solanum Khasianum </i> Clarke. In Vitro Cellular & Developmental Biology - Plant, 41(3): 291-295.
KREFT, S., ZEL, J., PUKL, M., UMEK, A. and STRUKELJ, B. 2000. Non-Aqueous Capillary Electrophoresis for the Simultaneous Analysis of Solasodine and Solasonine. Phytochemical Analysis, 11(1): 37-40.
KURONEN, P., VÄÄNÄNEN, T. and PEHU, E. 1999. Reversed-Phase Liquid Chromatographic Separation and Simultaneous Profiling of Steroidal Glycoalkaloids and their Aglycones. J. of Chromatography A, 863(1): p. 25-35.
LEE, M.-H., CHENG, J.-J., LIN, C.-Y., CHEN, Y.-J. and LU, M.-K. 2007. Precursor-Feeding Strategy for the Production of Solanine, Solanidine and Solasodine by a Cell Culture of Solanum Lyratum. Process Biochemistry, 42(5): 899-903.
TRIVEDI, P. and PUNDARIKAKSHUDU, K. 2007. Novel TLC Densitometric Method for Quantification of Solasodine in Various <i> Solanum </i> Species, Market Samples and Formulations. Chromatographia, 65(3): 239-243.
WEISSENBERG, M. 2001. Isolation of Solasodine and other Steroidal Alkaloids and Sapogenins by Direct Hydrolysis-Extraction of Solanum Plants or Glycosides Therefrom. Phytochemistry, 58(3): 501-508.